Programmed for Vysis Probes

Validated protocols for Vysis probes are preprogrammedand incorporate a pre-warming slide “dip” to ensure that the critical 73°C target temperature is constantly maintained during washing. Users may also create custom protocols to meet special conditions for other probes.

Automates Day 1 Slide Processing

The Little Dipper® Processor can also be used to automate Day 1 sample processing. The system’s fine control of temperature and washing action makes it ideal for processing bone marrow and blood cells, FFPE and other FISH samples. Using additional baths, it performs deparaffinization, deproteinization, fixation and dehydration of up to 24 slides per batch.

Use it for aCGH Based Tests Too!

The Little Dipper® Processor for FISH is ready to use for processing microarrays for laboratories that offer aCGH as a diagnostic tool. The system automates the post-hybridization steps used with Agilent, Illumina, Roche NimbleGen, SignatureSelect® and self-spotted arrays.

Protocols for Pre and Post-Hyb FISH Processing

• FISH Pre-Hybridization Processing of Blood and Bone Marrow Cells
  
  
• FISH Pre-Hybridization Processing of FFPE Samples
  
  
• Post-Hybridization Processing of FISH Assays for Vysis TelVysion™, AneuVysion™, CEP® and LSI® Probes (1-12 slides)
  
  
• Post-Hybridization Processing of FISH Assays for Vysis TelVysion™, AneuVysion™, CEP® and LSI® Probes (up to 24 slides)
  

• GTG Banding of Chromosomes on Slides
  

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Automates Slide Processing Using Vysis® and Other Probes

Post-Hyb Process up to 24 Slides at One Time

The Little Dipper® Processor for FISH [Cat. #1080-70-1 (115V); 1080-70-2 (230V)] provides precisely controlled automation of the post-hybridization processing steps used with fluorescent in situ hybridization (FISH) methods. The robotic system controls wash agitation, time and buffer temperatures for processing batches of up to 24 slides following optimized protocols. The system eliminates messy Coplin jars, waterbaths and staining dishes used in manual methods, freeing up valuable technician time.

Accurate Temperature Control Improves Assay Reproducibility

Precise control of buffer temperatures is critical for obtaining consistent low backgrounds and high FISH probe signals. The Little Dipper® Processor quickly heats and then regulates wash buffer temperatures to ± 0.5°C from set point — performance far superior to water baths and hot plates. The instrument is designed for use by CLIA-certified labs with a built-in system for verifying and calibrating accurate buffer temperatures.

Simple Training and Operation

After filling baths, setting temperatures and activating stir bars, slides are loaded into either a 12 or 24-position rack and mounted onto the robotic arm. Dual washing action is acheived through the movement of the robotic arm and stir bar vortex. The instrument performs programmed wash and dry steps and holds the slides in the dark ready for counterstain and imaging. The simplicity of design makes training a snap and ensures consistent day-to-day results by different operators.