Little Dipper™ Processor for FISH
Automates Slide Processing Using Vysis® and Other Probes
The Little Dipper Processor for FISH [Cat. #1080-70-1 (115V); 1080-70-2 (220V)] provides precisely controlled automation of the post-hybridization processing steps used with fluorescent in situ hybridization (FISH) methods. The robotic system controls wash agitation, time and buffer temperatures for processing batches of up to 24 slides following optimized protocols. The system eliminates messy Coplin jars, waterbaths and staining dishes used in manual methods, freeing up valuable technician time.
Accurate Temperature Control Improves Assay Reproducibility
Precise control of buffer temperatures is critical for obtaining consistent low backgrounds and high FISH probe signals. The Little Dipper Processor quickly heats and then regulates wash buffer temperatures to ± 0.5°C from set point — performance far superior to water baths and hot plates. The instrument is designed for use by CLIA-certified labs with a built-in system for verifying and calibrating accurate buffer temperatures.
Simple Training and Operation
After filling baths, setting temperatures and activating stir bars, slides are loaded into either a 12 or 24-position rack and mounted onto the robotic arm. Dual washing action is acheived through the movement of the robotic arm and stir bar vortex. The instrument performs programmed wash and dry steps and holds the slides in the dark ready for counterstain and imaging. The simplicity of design makes training a snap and ensures consistent day-to-day results by different operators.
Programmed for Vysis Probes
Validated protocols for Vysis probes are preprogrammed incorporating a pre-warming slide “dip” to ensure that the critical 73°C target temperature is constantly maintained during washing. Users may also create custom protocols to meet special conditions for other probes.
Achieve high
signals and low
backgrounds with
Vysis or other
FISH probes.
Automates Day 1 Slide Processing
The Little Dipper Processor can also be used to automate Day 1 sample processing. The system’s fine control of temperature and washing action makes it ideal for processing FFPE and other FISH samples. Using additional baths, it performs deparaffinization, deproteinization, fixation and dehydration of up to 24 slides per batch.
Use it for aCGH Based Tests Too!
The Little Dipper Processor for FISH is ready to use for processing microarrays for laboratories that offer aCGH as a diagnostic tool. The system automates the post-hybridization steps used with Agilent, Illumina, Roche/NimbleGen, Signature-Select® and self-spotted arrays.
