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| A wide range of different methods are used for microarray sample labeling, hybridization and post-hybridization processing of self-spotted / in-house made arrays. All of the procedures share the common drawback of the use of a collection of laboratory equipment and supplies including heating blocks, staining dishes, stirrers, waterbaths etc. that need to be collected, cleaned, calibrated and assembled for each run. Once set up, the procedure is technically challenging with many sample and device handling steps and the need to monitor and time elevated temperature washes. The complexity of the method provides multiple entry points for variability in data and the potential for costly outright failures. SciGene instruments automate and simplify the entire workflow used with self-spotted microarrays from sample preparation through slide processing for improved test reliability and enhanced data quality. |  |
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TechnologySciGene provides a family of instruments that work together to automate the workflow used with self spotted microarray assays from sample isolation and labeling through array hybridization and post-hybridization processing. The ArrayPrep™ Target Preparation System is a reagent handling and incubation platform specially designed for amplifying/labeling and then loading DNA onto microarrays. The Mai Tai® Hybridization System accommodates robotic sample loading and applies bubble mixing technology during incubation of multiple slides for most array formats. The Little Dipper® Processor with NoZone® Workspace automates all of the commonly used methods for washing and drying of arrays in an ozone-safe environment presenting slides ready for scanning. |
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Automating the Microarray Workflow
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Case Study |
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Without the automation of the Little Dipper, we simply could not manually wash the high-throughput number of samples that we currently process." — Bruce J. Paster, Ph.D., The Forsyth Institute |
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Literature (PDF) |
Customers |
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