BlueGnome’s recommended manual labeling, hybridization and post-hybridization processing methods for its CytoChip™ aCGH-based tests use a collection of laboratory equipment and supplies including staining dishes, slide boxes, stirrers, Coplin jars, a waterbath, oven etc. that need to be collected, cleaned and assembled for each run. Once set up, the procedure is technically challenging with many handling steps including the use of seven different washes with careful monitoring of buffer temperatures. The complexity of the methods provides multiple entry points for variability that can lead to ambiguous patient results and a higher cost per test. SciGene instruments automate and simplify the complex and error-prone manual steps used with BlueGnome microarrays from sample preparation through slide processing for improved test reliability and lowered cost.

Technology

SciGene provides a family of instruments that work together to automate the workflow used with CytoChip aCGH assays from sample isolation and labeling through array hybridization and post-hybridization processing. The ArrayPrep™ Target Preparation System is a reagent handling and incubation platform specially designed for amplifying/labeling and then loading DNA onto microarrays. The Mai Tai® Hybridization System accommodates robotic sample loading of multiple arrays and applies bubble mixing technology that generates higher signals. The Little Dipper® Processor with NoZone® Workspace automates BlueGnome’s recommended protocols for washing and drying of arrays in an ozone-safe environment presenting slides ready for scanning.

Automating the Microarray Workflow

Literature (PDF)

• ArrayPrep™ Target Preparation System
  
  
• Little Dipper® for Self-Spotted Arrays
  
  
• Mai Tai™ Hybridization System
  
  
• Ozone Control Systems for Microarray Labs
  
  
• Pre and Post-Hyb Processing of BlueGnome CytoChips

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