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Agilent’s recommended methods of hybridization and post-hybridization processing of microarrays requires a collection of laboratory equipment and supplies including multiple SureHyb Chambers, staining dishes, waterbaths etc. that need to be collected, cleaned and assembled for each run. Once set up, the procedure is technically challenging with the need for handling many single slide chambers, monitoring and timing of critical wash buffer temperatures and a tedious slow rack withdrawal step from the final wash bath. The complexity of the method provides multiple entry points for variability in data and the potential for costly failures. SciGene instruments automate and simplify the complex and error-prone manual steps used with Agilent microarrays from sample preparation through slide processing for improved assay reliability and lowered cost. |
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TechnologySciGene provides a family of instruments that work together to automate the workflow used in Agilent microarray based assays from sample isolation and labeling through array hybridization and post-hybridization processing. The ArrayPrep™ Target Preparation System is a reagent handling and incubation platform specially designed for amplifying/labeling and then loading DNA onto microarrays. The Mai Tai® Hybridization System accommodates robotic sample loading and applies bubble mixing technology during incubation of multiple slides for all Agilent array formats. The Little Dipper® Processor with NoZone® Workspace automates Agilent’s recommended protocols for washing and drying of arrays in an ozone-safe environment presenting slides ready for scanning. |
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Automating the Microarray Workflow |
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Case Study |
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Literature (PDF) |
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