SciGeneAutomating Array Workflows

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Solutions for Cytogenetic Labs

Solutions for Cytogenetic Labs

Overview

   

Cytogenetic laboratories are facing the prospect of re-tooling their laboratories for performing aCGH-based tests. The complexity and vast number of manual steps used to prepare samples and process microarrays can lead to errors, resulting in inconclusive test results with a subsequently higher cost per test. Similarly, manual processing of FISH slides used in current cytogenetic tests can also lead to poor test results. SciGene instruments automate and simplify the complex and error-prone manual steps used with aCGH and FISH-based tests from sample preparation through slide processing, for improved test reliability and lower cost.


  SciGene Solutions for Cytogenetic Labs
   

Technology

FISH
The Little Dipper® Processor automates Day 2, post-hybridization processing of up to 24 FISH slides per batch. It provides true walk-away automation of all wash steps and precisely controls critical wash buffer temperatures following optimized protocols for Vysis and other probes. The system eliminates messy Coplin jars, waterbaths and staining dishes, frees up valuable technician time and generates slides with brighter signals and lower background for clearer and more confident interpretations.The Little Dipper® Processor can also be used for Day 1, pre-hybridization processing of FISH slides.

aCGH
SciGene has a family of instruments that work together to automate the workflow used in aCGH-based tests from sample isolation and labeling through array hybridization and post-hybridization processing. The ArrayPrep™ Target Preparation System is a reagent handling and incubation platform specially designed for purifying, labeling and loading DNA derived from patient samples onto microarrays. The Mai Tai® Hybridization System accommodates robotic sample loading and applies bubble mixing technology while incubating multiple slides for popular array types, including Agilent. The Little Dipper® Processor with NoZone® Workspace automates washing and drying microarrays in an ozone-safe environment to present slides ready for scanning.

 
     

Automating the Microarray Workflow Automating the Microarray Workflow

     

Case Study

       
David Ledbetter
  “The Emory Genetics Laboratory provides state of the art genetic testing services. Our laboratory is a leader in array based testing for genome-wide chromosomal imbalances and single gene analysis using the EmArray Cyto6000 and EmArray Dystrophin microarrays developed in our laboratory (Baldwin et al. (2008) Genet Med).

We are very interested in automating the workflow used with our EmArray platforms to improve test reliability and are working with SciGene in validating a suite of products they have developed. Recently, we successfully validated the Little Dipper® Processor for both EmArrays and FISH slide processing in our clinical laboratory and have found it to be a simple and useful piece of equipment. It has become very popular with our technicians who previously conducted these tasks manually.”

— Dr. David Ledbetter and Dr. Christa Lese Martin
Emory University


   
David Ledbetter      
       

Christa Lese Martin

     
Christa Lese Martin      
     

Literature (PDF)

 

CustomersSciGene cytogenetic customers

     
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