Overview

aCGH Workflow

Case Study

DNA Labeling and Purification

“I run a busy aCGH-based genetic testing laboratory where we process more than 250 patient samples each week using BAC and oligo microarrays. As our operation has grown, we have worked closely with SciGene to automate our laboratory workflow.

Little Dipper Processors with ozone control have been in operation for over two years and provide clean arrays ready for scanning without risk of ozone degradation. We are incorporating the new ArrayPrep Target Preparation system into our laboratory to automate the many steps normally used for DNA labeling and clean up. SciGene’s automation technology has enabled us to handle increasing sample volumes while improving reproducibility and throughput. SciGene has been a committed and responsive partner over the years.” — Kyle Sundin, Laboratory Manager
Signature Genomic Laboratories, LLC

The ArrayPrep® Target Preparation System is a robotic liquid handling and incubation system that automates DNA labeling and array hybridization setup methods widely used in microarray analysis.

• Prepares 8 to 96 DNA samples ready for hybridization
• Requires less than an hour hands-on time
• Features 3 plate heating/cooling positions with removable heated lids
• Pipettes from 1 to 250µl
• Performs single or eight channel dispensing (minimizes reagent use)
• Integrated plate gripper automates plate movement
• Compatible with the Mai Tai® Hybridization System
• Fully optimized for easy setup using validated protocol

Array Set Up and Mixing

• Hybridizes four slides in one cassette — holds eight cassettes or up to 32 slides
• Two piece cassette design reduces handling errors.
• Provides bubble mixing for higher signals.
• Compatible with ArrayPrep® System for automated sample loading.
• Solid door protects light sensitive dyes during hybridization.
• Rotates from 2 to 80 RPM with a jog feature for easy loading.
• Provides uniform temperature to ±0.1°C from set point.
• Regulates temperature from ambient +5 to 99°C.
• Easy temperature validation / calibration with digital thermometer.
• CLIA compliant.

Array Washing and Drying

Customers

• Ability to process up to 24 samples per batch.
• Controls critical wash temperatures to ±0.5 degree.
• Comes with pre-programmed validated protocols.
• Holds slides after processing in a low light environment prior to scanning.
• Operates in an ozone-safe environment to protect sensitive fluorescent samples from destruction that can distort results.
• Compatible with all slide based methods including microarrays and cytogenetic applications (FISH, G-Banding/karyotyping).
• Compatible with commercial microarray providers including Roche NimbleGen, Agilent, Illumina and BlueGnome, as well as self-spotted slides.
• CLIA compliant.

Many members of International Standards for Cytogenomic Arrays Consortium (ISCA) use SciGene technology to improve their workflows. Ask us for references.

Literature

• Automating the aCGH Workflow
  
• ArrayPrep® Target Preparation System
  
• Little Dipper® for Agilent Arrays
  
• Little Dipper® for FISH
  
• Mai Tai® Hybridization System
  
• Ozone Control Systems for Microarray Labs

Ready for a Quote?

(800) 342-2119
sales@scigene.com